Review



crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Addgene inc crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid
    Crispr Cas9 Genomic Engineering Idt Star Methods Rbns Primers Idt Star Methods Recombinant Dna Px458 Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4084 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm41932309-792-83-97
    Average 96 stars, based on 4084 article reviews
    crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Knock-Out:

    Article Title: Yeast Smy2 and its human homologs GIGYF1 and -2 regulate Cdc48/VCP function during transcription stress
    Article Snippet: .. To generateGIGYF 1 and 2 knock-out cells, MRC5VA cells were transfected with plasmid pX458 (Addgene) containing the indicated gRNA sequences (Benchling.com) using lipofectamine 3000 (Invitrogen, ThermoFisher Scientific). ..

    Transfection:

    Article Title: Yeast Smy2 and its human homologs GIGYF1 and -2 regulate Cdc48/VCP function during transcription stress
    Article Snippet: .. To generateGIGYF 1 and 2 knock-out cells, MRC5VA cells were transfected with plasmid pX458 (Addgene) containing the indicated gRNA sequences (Benchling.com) using lipofectamine 3000 (Invitrogen, ThermoFisher Scientific). ..

    Article Title: Allele editing and applications thereof
    Article Snippet: 2×106 activated T cells were electroporated with the Invitrogen Neon© Transfection System at the following conditions: voltage (1550 V), width (10 mS), pulses (3) (Invitrogen), 100 pl tip, buffer R (for all electroporations buffer R was used). .. Cells were transfected with 6.5 pg of empty plasmid px458 (Addgene plasmid number 48138) or the plasmids described in Figure legends and Suppl. .. Table 1. (Addgene plasmid numbers 82670-82677).

    Article Title: Allele editing and applications thereof
    Article Snippet: .. Cells were transfected with 6.5 pg of empty plasmid px458 (Addgene plasmid number 48138) or the plasmids comprising the dsDNA repair template. .. For HDR cells were co-transfected with 12 pg (or 1200 ng, 600 ng, 250 ng) HDR template (if plasmid) or 10 pl of 10 pM stock ssDNA template from (IDT).

    Article Title: Allele editing and applications thereof
    Article Snippet: 2×106 activated T cells were electroporated with the Amaxa Transfection System, T-020 program (for plasmid) or using Neon® Transfection System (ThermoFisher) at the following conditions: voltage (1600V), width (10 ms), pulses (3) 100 pl tip, buffer R (for RNPs). .. Cells were transfected with 6.5 pg of empty plasmid px458 (Addgene plasmid number: 48138) or crRNA:tracerRNA-Atto 550 (IDT) and Cas9 (Berkeley) complex. ..

    Plasmid Preparation:

    Article Title: Yeast Smy2 and its human homologs GIGYF1 and -2 regulate Cdc48/VCP function during transcription stress
    Article Snippet: .. To generateGIGYF 1 and 2 knock-out cells, MRC5VA cells were transfected with plasmid pX458 (Addgene) containing the indicated gRNA sequences (Benchling.com) using lipofectamine 3000 (Invitrogen, ThermoFisher Scientific). ..

    Article Title: Synthetic tunable promoters for flexible control of multi-gene expression in mammalian cells
    Article Snippet: Gently scraped and collected all colonies from plates and purified the plasmids using EndoFree Maxi Plasmid Kit (Tiangen, DP117) according to the manufacturer’s instructions, resulting in the construction of our STP library. .. Primer pair F_PX458 and R_PX458 were used to amplify the U6 promoter and gRNA scaffold sequences from plasmid PX458 (Addgene, #48138). .. The amplified fragments were then integrated upstream of the CMV promoter in the SP-dCas9-VPR plasmid (Addgene, #63798) using the NEBuilder HiFi DNA Assembly Master Mix (NEB, #M5520) to generate the plasmid pCA (this study, ).

    Article Title: Enhanced Notch dependent gliogenesis and delayed physiological maturation underlie neurodevelopmental defects in Lowe syndrome
    Article Snippet: .. The two sgRNA fragments under the control of their independent U6 promoters were cloned into the plasmid PX458, pSpCas9(BB)-2A-GFP, (Addgene #48138) by Gibson assembly. ..

    Article Title: Allele editing and applications thereof
    Article Snippet: 2×106 activated T cells were electroporated with the Invitrogen Neon© Transfection System at the following conditions: voltage (1550 V), width (10 mS), pulses (3) (Invitrogen), 100 pl tip, buffer R (for all electroporations buffer R was used). .. Cells were transfected with 6.5 pg of empty plasmid px458 (Addgene plasmid number 48138) or the plasmids described in Figure legends and Suppl. .. Table 1. (Addgene plasmid numbers 82670-82677).

    Article Title: Allele editing and applications thereof
    Article Snippet: .. Cells were transfected with 6.5 pg of empty plasmid px458 (Addgene plasmid number 48138) or the plasmids comprising the dsDNA repair template. .. For HDR cells were co-transfected with 12 pg (or 1200 ng, 600 ng, 250 ng) HDR template (if plasmid) or 10 pl of 10 pM stock ssDNA template from (IDT).

    Article Title: Enhanced Notch dependent gliogenesis and delayed physiological maturation underlie neurodevelopmental defects in Lowe syndrome.
    Article Snippet: .. The two sgRNA fragments under the control of their independent U6 promoters were cloned into the plasmid PX458, pSpCas9(BB)-2A-GFP, (Addgene #48138) by Gibson assembly. ..

    Article Title: Allele editing and applications thereof
    Article Snippet: 2×106 activated T cells were electroporated with the Amaxa Transfection System, T-020 program (for plasmid) or using Neon® Transfection System (ThermoFisher) at the following conditions: voltage (1600V), width (10 ms), pulses (3) 100 pl tip, buffer R (for RNPs). .. Cells were transfected with 6.5 pg of empty plasmid px458 (Addgene plasmid number: 48138) or crRNA:tracerRNA-Atto 550 (IDT) and Cas9 (Berkeley) complex. ..

    Control:

    Article Title: Enhanced Notch dependent gliogenesis and delayed physiological maturation underlie neurodevelopmental defects in Lowe syndrome
    Article Snippet: .. The two sgRNA fragments under the control of their independent U6 promoters were cloned into the plasmid PX458, pSpCas9(BB)-2A-GFP, (Addgene #48138) by Gibson assembly. ..

    Article Title: Enhanced Notch dependent gliogenesis and delayed physiological maturation underlie neurodevelopmental defects in Lowe syndrome.
    Article Snippet: .. The two sgRNA fragments under the control of their independent U6 promoters were cloned into the plasmid PX458, pSpCas9(BB)-2A-GFP, (Addgene #48138) by Gibson assembly. ..

    Clone Assay:

    Article Title: Enhanced Notch dependent gliogenesis and delayed physiological maturation underlie neurodevelopmental defects in Lowe syndrome
    Article Snippet: .. The two sgRNA fragments under the control of their independent U6 promoters were cloned into the plasmid PX458, pSpCas9(BB)-2A-GFP, (Addgene #48138) by Gibson assembly. ..

    Article Title: Enhanced Notch dependent gliogenesis and delayed physiological maturation underlie neurodevelopmental defects in Lowe syndrome.
    Article Snippet: .. The two sgRNA fragments under the control of their independent U6 promoters were cloned into the plasmid PX458, pSpCas9(BB)-2A-GFP, (Addgene #48138) by Gibson assembly. ..

    other:

    Article Title: Microbial cancer immunotherapy reprograms hematopoiesis to enhance myeloid-driven anti-tumor immunity.
    Article Snippet: Article Microbial cancer immunotherapy reprograms hematopoiesis to enhance myeloid-driven antitumor immunity



    Similar Products

    96
    Addgene inc crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid
    Crispr Cas9 Genomic Engineering Idt Star Methods Rbns Primers Idt Star Methods Recombinant Dna Px458 Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm41932309-792-83-97
    Average 96 stars, based on 1 article reviews
    crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Addgene inc plasmid
    Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pmc13052343-1653-4-2
    Average 96 stars, based on 1 article reviews
    plasmid - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Addgene inc px458
    Px458, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pmc13052343-1653-0-2
    Average 96 stars, based on 1 article reviews
    px458 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    93
    Addgene inc 2x px458 pspcas9 bb 2a gfp
    2x Px458 Pspcas9 Bb 2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/phREX1-Luc+(Plasmid+%2317222)/pm41922518-508-12-13
    Average 93 stars, based on 1 article reviews
    2x px458 pspcas9 bb 2a gfp - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    96
    Addgene inc fluorescent protein gfp
    Fluorescent Protein Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/us12590323-392-45-48
    Average 96 stars, based on 1 article reviews
    fluorescent protein gfp - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Addgene inc mlmnb1 grna into pspcas9 bb 2a gfp
    Mlmnb1 Grna Into Pspcas9 Bb 2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm41917260-239-13-18
    Average 96 stars, based on 1 article reviews
    mlmnb1 grna into pspcas9 bb 2a gfp - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    94
    Addgene inc px458 plasmid carrying sgrna 749a 20nt
    Px458 Plasmid Carrying Sgrna 749a 20nt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/pC3_NLS_GFP_GST_RevNES+(Plasmid+%2345855)/us12590323-392-28-48
    Average 94 stars, based on 1 article reviews
    px458 plasmid carrying sgrna 749a 20nt - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    96
    Addgene inc bbsi digested plasmid pspcas9bb 2a gfp px458
    Bbsi Digested Plasmid Pspcas9bb 2a Gfp Px458, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm41910145-319-12-16
    Average 96 stars, based on 1 article reviews
    bbsi digested plasmid pspcas9bb 2a gfp px458 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Addgene inc pspcas9 2a gfp vector
    Pspcas9 2a Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm41931969-80-12-14
    Average 96 stars, based on 1 article reviews
    pspcas9 2a gfp vector - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Addgene inc size
    Size, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+px458/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm41911287-57-11-12
    Average 96 stars, based on 1 article reviews
    size - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results